How to Read a Protein FASTA Record
Identify the FASTA header and sequence lines, then prepare a single protein record for analysis in ResidueBench.
Read the header and the sequence separately
A FASTA record begins with a definition line. Put > at the start of that line, followed by an identifier. Place the one-letter amino acid sequence on the following lines.
>example_peptide
ac
DResidueBench reads this as ACD. It leaves the header out of the calculation, ignores whitespace within the sequence, and normalizes lowercase ASCII letters to uppercase.
Prepare one record for a calculation
If your file has several headers, paste one complete record at a time. You can also paste just the sequence. The calculators accept up to 10,000 residues using the 20 standard one-letter codes. Ambiguous codes such as X, gaps, stop markers, and modification annotations trigger an input error.
To check all records together, open the Protein FASTA Validator.